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human breast cancer cell line bt549  (ATCC)


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    ATCC human breast cancer cell line bt549
    Human Breast Cancer Cell Line Bt549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2985 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+cancer+cell+line+bt549/BT-549/pm41740339-52-16-42
    Average 99 stars, based on 2985 article reviews
    human breast cancer cell line bt549 - by Bioz Stars, 2026-10
    99/100 stars

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    Multiple Displacement Amplification:

    Article Title: Oridonin downregulates PD-L1 expression and promotes anti-tumor immunity via inhibiting NLRP3 and STAT3.
    Article Snippet: Blocking the programmed death-1 (PD-1)/programmed cell death ligand-1(PD-L1) axis has become an attractive treatment strategy for cancer immunotherapy.. Small molecules are promising agents for targeting the PD-1/PDL1 axis to enhance immunotherapy.. Here, we identified a natural product, oridonin, that can significantly reduce the abundance of PD-L1 in various cancer cells and enhance the cytotoxicity of stimulated Jurkat T-cells against cancer cells at submicromolar levels.

    Article Title: UBE2M forms a positive feedback loop with estrogen receptor to drive breast cancer progression and drug resistance
    Article Snippet: .. The human breast cell lines MCF7, T47D and MDA-MB-231 were obtained from the National Collections of Authenticated Cell Cultures, while the human breast cancer cell line BT549 and the human renal epithelial cell line 293T were obtained from the American Type Culture Collection. ..

    Cell Culture:

    Article Title: Paclitaxel drives TREM2 + macrophage expansion underlying its inferior therapeutic efficacy compared to Nab-paclitaxel
    Article Snippet: Human embryonic kidney cell line HEK293T (ATCC, CRL-3216), human breast cancer cell line SUM159 (CLS Cell Lines service, 305116), mouse melanoma cell lines B16-F10 (ATCC, CRL-6475) and mouse macrophage cell line Raw264.7 (ATCC, TIB-71) were cultured in Dulbecco’s Modified Eagle Medium (DMEM). .. Human breast cancer cell line BT549 (ATCC, HTB-122), Mouse breast cancer cell line 4T1-Luc (ATCC, CRL-2539-LUC2) and human macrophage cell lines THP1 (ATCC, TIB-202) were cultured in RPMI-1640 medium. .. Human breast cancer cell line MDA-MB-231(ATCC, CRM-HTB-26) was cultured in L15 medium.

    Article Title: Pyruvate Kinase M2-Responsive Release of Paclitaxel and Indoleamine 2,3-Dioxygenase Inhibitor for Immuno-Chemotherapy of Nonsmall Cell Lung Cancer.
    Article Snippet: .. Circular Dichroism (CD) Measurement: Using a CD spectrometer (Applied Photophysics), all 1.0 wt% hydrogels (Gel Nap-T and GP@Gel NapT) were measured at 20 °C and elevated temperatures, spanning the wavelength range of 280−190 nm, with a path length of 1.0 mm, scan speed of 50 nm min−1, and a response time of 2.0 s. Cell Culture: Human lung cancer cell lines A549, NCIH1975, NCIH466, murine lung cancer cell lines CMT167 and Lewis, human breast cancer cell line BT549, human glioblastoma cell line U251, and murine glioblastoma cell line GL261 were all obtained from American Type Culture Collection (ATCC). .. NCM460 cells (human colonic epithelial), HT29 cells (human colorectal carcinoma), MDAMB231 cells (human breast cancer), and 4T1 cells (murine breast cancer) were kindly gifted by Prof. Wang at China Pharmaceutical University, Nanjing, China.

    Article Title: Leader cells mechanically respond to aligned collagen architecture to direct collective migration
    Article Snippet: .. Tumor organoids or human breast cancer cell line BT549 (ATCC) in 3D aligned or random collagen gels were cultured for 3–6 days until invasive at 37°C. ..

    Article Title: Leader cells mechanically respond to aligned collagen architecture to direct collective migration.
    Article Snippet: .. Tumor organoids or human breast cancer cell line BT549 (ATCC) in 3D aligned or random collagen gels were cultured for 3–6 days until invasive at 37 ̊C. ..

    Circular Dichroism:

    Article Title: Pyruvate Kinase M2-Responsive Release of Paclitaxel and Indoleamine 2,3-Dioxygenase Inhibitor for Immuno-Chemotherapy of Nonsmall Cell Lung Cancer.
    Article Snippet: .. Circular Dichroism (CD) Measurement: Using a CD spectrometer (Applied Photophysics), all 1.0 wt% hydrogels (Gel Nap-T and GP@Gel NapT) were measured at 20 °C and elevated temperatures, spanning the wavelength range of 280−190 nm, with a path length of 1.0 mm, scan speed of 50 nm min−1, and a response time of 2.0 s. Cell Culture: Human lung cancer cell lines A549, NCIH1975, NCIH466, murine lung cancer cell lines CMT167 and Lewis, human breast cancer cell line BT549, human glioblastoma cell line U251, and murine glioblastoma cell line GL261 were all obtained from American Type Culture Collection (ATCC). .. NCM460 cells (human colonic epithelial), HT29 cells (human colorectal carcinoma), MDAMB231 cells (human breast cancer), and 4T1 cells (murine breast cancer) were kindly gifted by Prof. Wang at China Pharmaceutical University, Nanjing, China.



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    human breast cancer cell line bt549  (ATCC)
    99
    ATCC human breast cancer cell line bt549
    Human Breast Cancer Cell Line Bt549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+cancer+cell+line+bt549/BT-549/pm41740339-52-16-42
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    human breast cancer cell lines bt549  (ATCC)
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    ATCC human breast cancer cell lines bt549
    PAK2 expression and cluster formation in breast cancer cell lines. a mRNA expression levels of PAK2 in breast cancer cell lines and the normal mammary epithelial cell line MCF10A. b – f Quantification of cell cluster formation in four breast cancer cell lines under suspension culture conditions at 12, 24, and 48 h. g Immunoblot analysis of total PAK2 and phospho-PAK2 (Ser141) levels in <t>BT549,</t> MDA-MB-231, SKBR3, and AU565 breast cancer cells. SKBR3 cells were used as the reference (set to 1) for relative expression
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    bt549 human breast cancer cell line  (ATCC)
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    ATCC bt549 human breast cancer cell line
    PAK2 expression and cluster formation in breast cancer cell lines. a mRNA expression levels of PAK2 in breast cancer cell lines and the normal mammary epithelial cell line MCF10A. b – f Quantification of cell cluster formation in four breast cancer cell lines under suspension culture conditions at 12, 24, and 48 h. g Immunoblot analysis of total PAK2 and phospho-PAK2 (Ser141) levels in <t>BT549,</t> MDA-MB-231, SKBR3, and AU565 breast cancer cells. SKBR3 cells were used as the reference (set to 1) for relative expression
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    		  Buy from Supplier
    cell culture human breast cancer cell lines bt549  (ATCC)
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    ATCC cell culture human breast cancer cell lines bt549
    PAK2 expression and cluster formation in breast cancer cell lines. a mRNA expression levels of PAK2 in breast cancer cell lines and the normal mammary epithelial cell line MCF10A. b – f Quantification of cell cluster formation in four breast cancer cell lines under suspension culture conditions at 12, 24, and 48 h. g Immunoblot analysis of total PAK2 and phospho-PAK2 (Ser141) levels in <t>BT549,</t> MDA-MB-231, SKBR3, and AU565 breast cancer cells. SKBR3 cells were used as the reference (set to 1) for relative expression
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    PAK2 expression and cluster formation in breast cancer cell lines. a mRNA expression levels of PAK2 in breast cancer cell lines and the normal mammary epithelial cell line MCF10A. b – f Quantification of cell cluster formation in four breast cancer cell lines under suspension culture conditions at 12, 24, and 48 h. g Immunoblot analysis of total PAK2 and phospho-PAK2 (Ser141) levels in BT549, MDA-MB-231, SKBR3, and AU565 breast cancer cells. SKBR3 cells were used as the reference (set to 1) for relative expression

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: PAK2 expression and cluster formation in breast cancer cell lines. a mRNA expression levels of PAK2 in breast cancer cell lines and the normal mammary epithelial cell line MCF10A. b – f Quantification of cell cluster formation in four breast cancer cell lines under suspension culture conditions at 12, 24, and 48 h. g Immunoblot analysis of total PAK2 and phospho-PAK2 (Ser141) levels in BT549, MDA-MB-231, SKBR3, and AU565 breast cancer cells. SKBR3 cells were used as the reference (set to 1) for relative expression

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Expressing, Suspension, Western Blot

    PAK2 promotes cell cluster formation. a , b Immunoblot analysis showing PAK2 expression in BT549 cells after overexpression or knockdown. c Representative images and quantification of cluster formation in BT549 cells transfected with control vector (Con) or lenti-PAK2 (OE-PAK2). d Representative images and quantification of cluster formation in BT549 cells transfected with control siRNA (siCon) or siPAK2-1

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: PAK2 promotes cell cluster formation. a , b Immunoblot analysis showing PAK2 expression in BT549 cells after overexpression or knockdown. c Representative images and quantification of cluster formation in BT549 cells transfected with control vector (Con) or lenti-PAK2 (OE-PAK2). d Representative images and quantification of cluster formation in BT549 cells transfected with control siRNA (siCon) or siPAK2-1

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Western Blot, Expressing, Over Expression, Knockdown, Transfection, Control, Plasmid Preparation

    Effects of PAK2 on breast cancer cell progression. a , b Colony formation assays performed in BT549 cells with PAK2 overexpression or knockdown. c , d Wound healing assays evaluating the migration of BT549 cells with PAK2 overexpression or knockdown. e Transwell assays evaluating the invasive capacity of BT549 cells with PAK2 overexpression or knockdown

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: Effects of PAK2 on breast cancer cell progression. a , b Colony formation assays performed in BT549 cells with PAK2 overexpression or knockdown. c , d Wound healing assays evaluating the migration of BT549 cells with PAK2 overexpression or knockdown. e Transwell assays evaluating the invasive capacity of BT549 cells with PAK2 overexpression or knockdown

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Over Expression, Knockdown, Migration

    PAK2 mediates cell cluster formation via E-cadherin phosphorylation. a , b GSEA showing the association between PAK2 and the adherens junction pathway. c , d Immunoblot analysis of E-cadherin and phosphorylated E-cadherin (Ser840) in BT549 cells after PAK2 overexpression or knockdown

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: PAK2 mediates cell cluster formation via E-cadherin phosphorylation. a , b GSEA showing the association between PAK2 and the adherens junction pathway. c , d Immunoblot analysis of E-cadherin and phosphorylated E-cadherin (Ser840) in BT549 cells after PAK2 overexpression or knockdown

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Phospho-proteomics, Western Blot, Over Expression, Knockdown

    PAK2 interacts and colocalizes with E-cadherin. a Protein docking map and binding site of PAK2 for E-cadherin. The yellow color represents PAK2, and the blue color represents E-cadherin. b IF staining illustrating the colocalization of PAK2 and E-cadherin in MCF10A and BT549. c Co-IP analysis showing the association between PAK2 and E-cadherin in breast cancer cells

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: PAK2 interacts and colocalizes with E-cadherin. a Protein docking map and binding site of PAK2 for E-cadherin. The yellow color represents PAK2, and the blue color represents E-cadherin. b IF staining illustrating the colocalization of PAK2 and E-cadherin in MCF10A and BT549. c Co-IP analysis showing the association between PAK2 and E-cadherin in breast cancer cells

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Binding Assay, Staining, Co-Immunoprecipitation Assay

    FRAX597 inhibits PAK2 activity and CTC cluster formation. a CCK-8 assay evaluating the viability of BT549 after 48 h treatment with FRAX597. b Immunoblot analysis of total PAK2 and phosphorylated PAK2 protein levels in BT549 treated with 2.5 or 5 µM FRAX597 for 24 and 48 h. c Representative images and quantification of BT549 cell aggregation after 24 h FRAX597 treatment. d Immunoblot analysis of total and phosphorylated PAK2, and total and phosphorylated E-cadherin in BT549 treated with 2.5 or 5 µM FRAX597 for 24 h

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: FRAX597 inhibits PAK2 activity and CTC cluster formation. a CCK-8 assay evaluating the viability of BT549 after 48 h treatment with FRAX597. b Immunoblot analysis of total PAK2 and phosphorylated PAK2 protein levels in BT549 treated with 2.5 or 5 µM FRAX597 for 24 and 48 h. c Representative images and quantification of BT549 cell aggregation after 24 h FRAX597 treatment. d Immunoblot analysis of total and phosphorylated PAK2, and total and phosphorylated E-cadherin in BT549 treated with 2.5 or 5 µM FRAX597 for 24 h

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Activity Assay, CCK-8 Assay, Western Blot

    FRAX597 suppresses breast cancer progression. a – c Effects of FRAX597 on BT549 cell proliferation, migration and invasion. d Schematic diagram of in vivo experimental design. e , f Tumor volume, tumor weight and body weight measurements of nude mice treated with FRAX597

    Journal: Breast Cancer Research : BCR

    Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

    doi: 10.1186/s13058-025-02199-z

    Figure Lengend Snippet: FRAX597 suppresses breast cancer progression. a – c Effects of FRAX597 on BT549 cell proliferation, migration and invasion. d Schematic diagram of in vivo experimental design. e , f Tumor volume, tumor weight and body weight measurements of nude mice treated with FRAX597

    Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

    Techniques: Migration, In Vivo